ANTIOXIDANT AND ANALGESIC ACTIVITY OF TOTAL FLAVONOIDS OF FORSSKAOLEA TENACISSIMA.

JPUMHS;2024:14:04,22-31 . http://doi.org/10.46536/jpumhs/2024/14.04.557

Abstract

BACKGROUND: Total flavonoids of Forsskaolea tenacissima was screened for possible antioxidant
and analgesic activities. It was an attempt to isolate total flavonoids from the aerial parts of plant and
subject it for further pharmacological screenings. METHODS: Whole plant of Forsskaolea
tenacissima was collected from Institute of basic medical sciences, Khyber medical university and the
voucher specimen was sent in to the pharmacology department. Hot plate method, writhing method
and tail immersion methods were used to investigate the possible analgesic activity and DPPH was
used for the determination of antioxidant activity of Forsskaolea tenacissima while ascorbic acid
was used as a reference. RESULTS: Mice were divided into six groups and the lethality was zero
percent in animals of first three groups while there was 25 %, 50 % and 100 % lethality in animals of
group 4 to group 6 respectively. Total flavonoids of Forsskaolea tenacissima has analgesic activity
which is ascertained by the hot plate, writhing, and tail immersion procedures. The antioxidant
activity was less than the EC50, or 37.20 percent, at a concentration of 62.5µg/ml. Which gradually
increases and it reached to 74.10 % at 1000 µg/ml which shows that the plant has antioxidant
activity. CONCLUSION: The study shows that the total flavonoids of Forsskaolea tenacissima has
antioxidant activity as well as analgesic activity mediated through Hot plate method, writhing
method and tail immersion methods. Crude methanolic extract's initial phytochemical tests that the
plant includes flavonoids, which may be what give it its antioxidant properties.