ACUTE TOXICITY AND RELAXANT ACTIVITY OF TOTAL FLAVONOIDS OF FORSSKAOLEA TENACISSIMA.

JPUMHS;2024:14:04,60-70 .http://doi.org/10.46536/jpumhs/2024/14.04.561

Abstract

BACKGROUND: Abdominal spasms have historically been treated with the whole plant of
Forsskaolea tenacissima. Thus, the study's goals were to identify the safe dosage range,
identify the mechanism or mechanisms behind Forsskaolea tenacissima's therapeutic usage
for gastrointestinal spasms, and extract the plant's total flavonoids. METHODS:
Forsskaolea tenacissima's total flavonoids were examined for potential antispasmodic
efficacy in isolated rabbit jejunal preparations after an acute toxicity research was conducted
to establish the safe dosage range prior to in vivo tests. RESULTS: With comparable EC50
values of 4.22 ± 0.849 mg/ml and 0.607 ± 0.0306 mg/ml, total flavonoids from Forsskaolea
tenacissima decreased both spontaneous and high K+-induced contractions in isolated rabbit
jejunal preparations. The concentrations used were 0.01, 0.03, 0.1, 0.3, 1.0, 3.0, 5.0, and 10.0
mg/ml. The mechanism was verified by constructing a calcium curve in the decalcified rabbit
jejunal preparation with and without verapamil 0.1, 0.3 µm. The EC50 value of the total
flavonoids in Forsskaolea tenacissima is -2.55 ± 0.00 at 0.1 mg/ml, whereas the control has
an EC50 value of -2.80 ± 0.00. In the presence and absence of 0.1 µM verapamil, the
corresponding EC50 Log Ca++ M values are 1.71 ± 0.07 and -2.45 ± 0.00, respectively. This
suggests that the total flavonoids of Forsskaolea tenacissima follow voltage-gated calcium
channels for calcium influx since the right shift of Verapamil and the test sample's total
flavonoids right shifts are comparable. CONCLUSION: According to the study,
Forsskaolea tenacissima's total flavonoids have antispasmodic action that may be mediated
via voltage-gated Ca++ channel blockage, and the safe dose is 100 mg/kg. This gives the
plant a solid pharmacological foundation for its potential medical application in treating
intestinal spasm.